rabbit polyclonal antibodies against chop  (ABclonal Biotechnology)

Journal: Bioscience Reports

Article Title: PKCδ silencing alleviates saturated fatty acid induced ER stress by enhancing SERCA activity

doi: 10.1042/BSR20170869

Figure Lengend Snippet: ( A ) L02 cells were incubated with BSA or 0.5 mM PA for 16 h, and the morphological changes were observed by TEM. Arrowheads indicate the rough ER. ( B ) Western blotting analysis showed PKCδ and p-PKCδ expression at the indicated time with or without PA treatment. ( C ) Western blotting analysis indicated Bip, ATF6, IRE-1, and CHOP expression in L02 cell treated with BSA or PA for 16 h. All the data repeated three times.

Article Snippet: The primary antibodies included rabbit polyclonal antibody against PKCδ, p-PKCδ, Bip, CHOP (1:1000; Cell Signaling Technology, U.S.A.), β-actin (1:1000; Biosynthesis Biotechnology, Beijing, China), ATF6 and goat polyclonal antibody IRE-I (1:1000; Origene Technology, U.S.A.).

Techniques: Incubation, Western Blot, Expressing

Journal: Bioscience Reports

Article Title: PKCδ silencing alleviates saturated fatty acid induced ER stress by enhancing SERCA activity

doi: 10.1042/BSR20170869

Figure Lengend Snippet: L02 cells were transfected with PKCδ siRNA (siPKCδ) or negative control siRNA (siNC) for 24 h and then treated with BSA or 0.5 mM PA for an additional 16 h. Real-time PCR showed the expression of PKCδ ( A ), Bip and CHOP ( C ). ( B ) Western blots showed the expression of PKCδ and p-PKCδ. ( D ) Western blots showed the expression of Bip and CHOP in L02 cells with siPKCδ or other indicated treatments. All the data repeated three times. Data represent the mean ± S.D. ** P <0.01, *** P <0.001. Abbreviation: N.S., no significance.

Article Snippet: The primary antibodies included rabbit polyclonal antibody against PKCδ, p-PKCδ, Bip, CHOP (1:1000; Cell Signaling Technology, U.S.A.), β-actin (1:1000; Biosynthesis Biotechnology, Beijing, China), ATF6 and goat polyclonal antibody IRE-I (1:1000; Origene Technology, U.S.A.).

Techniques: Transfection, Negative Control, Real-time Polymerase Chain Reaction, Expressing, Western Blot